What kind of stain is methylene blue

History In addition to the staining of microorganisms, Methylene Blue was demonstrated by Harris in to be an effective stain for the enumaration of leukocytes in mucus of faeces. Microbiology on the go. An initiative by Dept. Medical Microbiology and Infectious diseases. Disclaimer RSS Feeds. Methylene Blue Staining. Application in Wine Microbiology: Methylene blue staining is useful in determining cell mortality. References: Brooks, M.

The Scientific Monthly. Glick, B. Your browser does not have JavaScript enabled and some parts of this website will not work without it. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. Methylene Blue Stain Solution ab has been optimized to give outstanding results when used in various procedures such as the Ziehl-Neelsen, Fite's, and Gram stain.

The stain is also suggested for use in staining a variety of bacteria Corynebacterium diphtheria, Haemophilus influenzae, Neisseria, etc. This reagent produces dark blue nuclei with very light blue cytoplasm.

Apply adequate Methylene Blue Solution to completely cover tissue section and incubate for minutes. Alternative: After staining, rinse briefly in distilled water dips , allow slide to air dry, clear in xylene or xylene replacement, and mount in synthetic resin.

Find more kits and reagents in the special stains guide , or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide. Clin Ophthalmol. Published online Oct Katsuji Kiuchi. Author information Copyright and License information Disclaimer.

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This article has been cited by other articles in PMC. Results Histopathological images of the conjunctival epithelial cells and neutrophils in eye discharges were dyed blue and the nuclei were stained more intensely blue. Keywords: alkaline methylene blue, supravital staining, discharge, anterior segment infections. Introduction Conjunctivitis is one of the most common diseases encountered by ophthalmologists in their daily practice.

Table 1 Summary of patient diagnosis and characteristics. Case Age years Sex Diagnosis Microscopic finding 1 0. Open in a separate window. Results Photomicrographs of the bacteria in the eye discharge of a patient with chronic conjunctivitis are shown in Figure 1A and B.

Figure 1. Figure 2. Figure 3. Figure 4. Figure 5. Discussion Smear histopathology and bacterial culture tests are being used more commonly by ophthalmologists for the diagnosis of corneal and conjunctival infections.

Figure 6. Footnotes Disclosure The author reports no conflicts of interest in this work. References 1. Yanoff M, Fine BS. Ocular Pathology. St Louis: Mosby;